Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Apr 27, 2026

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform
09:41

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Published on: February 25, 2021

24.6K

Liposomal spherical nucleic acids.

Resham J Banga1, Natalia Chernyak, Suguna P Narayan

  • 1International Institute of Nanotechnology, ‡Department of Chemical and Biological Engineering, §Department of Chemistry, and ⊥Department of Biomedical Engineering, Northwestern University , 2145 Sheridan Road, Evanston, Illinois 60208, United States.

Journal of the American Chemical Society
|July 2, 2014
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Role of Structural Enthalpy in Spherical Nucleic Acid Hybridization.

Journal of the American Chemical Society·2018
Same author

The Weak-Link Approach to the Synthesis of Inorganic Macrocycles.

Angewandte Chemie (International ed. in English)·2018
Same author

Ligand Design for Electrochemically Controlling Stoichiometric and Catalytic Reactivity of Transition Metals.

Angewandte Chemie (International ed. in English)·2018
Same author

The Electrical Properties of Gold Nanoparticle Assemblies Linked by DNA.

Angewandte Chemie (International ed. in English)·2018
Same author

Directed Assembly of Periodic Materials from Protein and Oligonucleotide-Modified Nanoparticle Building Blocks.

Angewandte Chemie (International ed. in English)·2018
Same author

PLGA Spherical Nucleic Acids.

Advanced materials (Deerfield Beach, Fla.)·2018
Same journal

A Ni-Mediated Cross-Coupling Approach to Deuterated <sup>18</sup>F- Fluoromethylated (Hetero)arenes.

Journal of the American Chemical Society·2026
Same journal

Efficient Light-Driven CO<sub>2</sub> Capture and Reversible Release Enabled by Metastable Photoacid-Decorated Metal-Organic Frameworks.

Journal of the American Chemical Society·2026
Same journal

In Situ Raman Spectroscopy Reveals the Dynamic Evolution and Ethanol Dependence of SEI Structure in Li-Mediated N<sub>2</sub> Reduction Reaction.

Journal of the American Chemical Society·2026
Same journal

Solvent Esterification and Stoichiometric Control in Ambient-Grown FAPbI<sub>3</sub> Single-Crystal Solar Cells.

Journal of the American Chemical Society·2026
Same journal

Unlocking Azulene Functionalization via Strain-Induced Azulyne Intermediates.

Journal of the American Chemical Society·2026
Same journal

An Oxazine-Locked Covalent Organic Framework by a Tandem Pinner/Schiff Base Reaction for Hydrogen Peroxide Photosynthesis.

Journal of the American Chemical Society·2026
See all related articles

Researchers developed new metal-free spherical nucleic acid nanostructures using liposomes and DNA. These novel nanostructures enhance cellular uptake and gene regulation, offering a promising tool for biomedical applications.

Area of Science:

  • Biotechnology
  • Nanomedicine
  • Molecular Biology

Background:

  • Spherical nucleic acid (SNA) nanostructures offer unique properties for biomedical applications.
  • Liposomes are FDA-approved lipid-based nanoparticles widely used in drug delivery.
  • Developing metal-free nanostructures is desirable to avoid potential toxicity.

Purpose of the Study:

  • To synthesize and characterize a novel class of metal-free spherical nucleic acid nanostructures.
  • To evaluate the cellular internalization and gene regulation capabilities of these nanostructures.
  • To explore the potential of these nanostructures in SKOV-3 cells.

Main Methods:

  • Synthesis of liposomal cores (30 nm) using 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC).
  • Functionalization of liposome surfaces with DNA strands modified with tocopherol tails.

More Related Videos

Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes
10:33

Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes

Published on: July 23, 2016

10.4K
Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
08:29

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

9.3K

Related Experiment Videos

Last Updated: Apr 27, 2026

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform
09:41

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Published on: February 25, 2021

24.6K
Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes
10:33

Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes

Published on: July 23, 2016

10.4K
Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
08:29

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

9.3K
  • Assessment of nanostructure stability, cellular uptake, and gene regulation in SKOV-3 cells.

    • Main Results:

    • Successfully synthesized metal-free spherical nucleic acid nanostructures with liposomal cores.
    • Tocopherol-modified DNA strands intercalated into the liposomal core via hydrophobic interactions, stabilizing the structure.
    • Demonstrated efficient cellular internalization and gene regulation activity in SKOV-3 cells.

    Conclusions:

    • A novel, metal-free spherical nucleic acid nanostructure class was developed using accessible materials.
    • The unique architecture enhances stability and facilitates cellular entry.
    • These nanostructures show promise for gene regulation applications in cancer cells.