Fluorescence2D: software for accelerated acquisition and analysis of two-dimensional fluorescence spectra

Area of Science:

• Spectroscopy
• Data Analysis Software

Background:

• Two-dimensional (2D) fluorescence spectroscopy provides rich information but can be time-consuming to acquire.
• Accelerated acquisition schemes, like triangular acquisition, offer faster data collection but require specialized analysis tools.

Purpose of the Study:

• To present Fluorescence2D, a novel, free software solution for analyzing 2D fluorescence spectra.
• To facilitate the processing and visualization of data acquired using accelerated triangular schemes.

Main Methods:

• The software integrates Python and MATLAB programming languages.
• It implements algorithms for converting triangular acquisition data into standard 2D spectra.
• Features include interactive display, 1D slice extraction, and versatile graphic output.

Main Results:

• Fluorescence2D successfully processes and visualizes 2D fluorescence spectra from triangular acquisition data.
• The software enables efficient extraction of 1D spectral slices at selected wavelengths.
• Output is available in multiple common graphic formats for further analysis and publication.

Conclusions:

• Fluorescence2D offers a user-friendly and efficient platform for researchers working with 2D fluorescence spectroscopy.
• The software streamlines the analysis workflow, making advanced spectroscopic techniques more accessible.