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Updated: Apr 27, 2026

Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
Published on: February 12, 2019
The application of KillerRed for acute protein inactivation in living cells
Timothy S Jarvela1, Adam D Linstedt
1Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania.
Abstract:
Generating loss of protein function is a powerful investigatory tool particularly if carried out on a physiologically relevant timescale in a live-cell fluorescent imaging experiment. KillerRed mediated chromophore assisted light inactivation (CALI) uses genetic encoding for specificity and light for acute inactivation that can also be spatially restricted. This unit provides protocols for setting up and carrying out properly controlled KillerRed experiments during live-cell imaging of cultured cells.

