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Updated: Apr 27, 2026
Culturing In Vivo-like Murine Astrocytes Using the Fast, Simple, and Inexpensive AWESAM Protocol
Published on: January 10, 2018
Differentiation of reactive-like astrocytes cultured on nanofibrillar and comparative culture surfaces
Volkan Müjdat Tiryaki1, Virginia M Ayres, Ijaz Ahmed
1Electronic & Biological Nanostructures Laboratory, Department of Electrical and Computer Engineering, 428 S Shaw Lane, Michigan State University, Engineering Building Room 2120, East Lansing, MI, 48824, USA. tiryakiv@msu.edu.
Aim:
To investigate the directive importance of nanophysical properties on the morphological and protein expression responses of dibutyryladenosine cyclic monophosphate (dBcAMP)-treated cerebral cortical astrocytes in vitro.
Materials & Methods:
Elasticity and work of adhesion characterizations of culture surfaces were performed using atomic force microscopy and combined with previous surface roughness and polarity results. The morphological and biochemical differentiation of dBcAMP-treated astrocytes cultured on promising nanofibrillar scaffolds and comparative culture surfaces were investigated by immunocytochemistry, colocalization, super resolution microscopy and atomic force microscopy. The dBcAMP-treated astrocyte responses were further compared with untreated astrocyte responses.
Results & Conclusion:
Nanofibrillar scaffold properties were shown to reduce immunoreactivity responses while poly-L-lysine-functionalized Aclar® (Ted Pella Inc., CA, USA) properties were shown to induce responses reminiscent of glial scar formation. The comparison study indicated that directive cues may differ in wound-healing versus quiescent situations.
