Related Experiment Video
Updated: Jun 18, 2026

Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats
Published on: December 7, 2021
Polyphasic taxonomic characterization of Brachybacterium netajii sp. nov., a metabolically versatile bacterium
Sk Aftabul Alam1,2, Debabrata Karmakar3, Biswajit Khan3
1Department of Botany, Netaji Mahavidyalaya, Arambagh, Hooghly, WB, India. aftabul1992@gmail.com.
Abstract:
A comprehensive polyphasic taxonomic strategy was applied to the systematic characterization of strain DNPG3T, which was isolated from the river Ganges, Hooghly, West Bengal, India. The Gram-positive, halotolerant, heavy-metal-tolerant strain exhibited the ability to degrade p-nitrophenol (PNP). Cellular fatty acid analysis revealed that the predominant components were anteiso-C15:0 (24.61%), C11:0 (21.06%), iso-C16:0 (11.89%), C16:0 (11.58%), and anteiso-C17:0 (11.24%). Notably, the presence of C11:0, C10:0 2-OH as major fatty acids differentiate strain DNPG3T from its closely related members of the genus Brachybacterium. The predominant respiratory quinone was identified as menaquinone-7 (MK-7). Analysis of 16S rRNA gene sequence indicated that B. zhongshanense strain JBT was the closest relative of DNPG3T, sharing 97.08% sequence similarity. Genome-based ANI value calculated using the EzBioCloud server revealed that B. zhongshanense JCM 15471T was the closest genomic relative (85.49%). These values were further substantiated by digital DNA-DNA hybridization (dDDH) estimates calculated using the GGDC server. Taxonomic assignment using the GTDB database further indicated that strain DNPG3T constitutes a previously unrecognized species within the genus Brachybacterium. Genome analysis of strain DNPG3T identified eleven genomic islands, along with a rich repertoire of 194 carbohydrate-active enzyme (CAZyme) families, comprising 95 glycoside hydrolases and 53 glycosyltransferases. In addition, five biosynthetic gene clusters were detected. Collectively, these genomic features indicate the involvement of horizontal gene transfer events and highlighted the pronounced metabolic versatility of the strain, underscoring its potential for industrial enzyme production and secondary metabolite biosynthesis. Pan-genome analysis further indicates that the Brachybacterium pan-genome is open, reflecting substantial genetic diversity and ongoing gene acquisition within the genus. Comprehensive biochemical, physiological, chemotaxonomic, and phylogenetic analyses supported the assignment of strain DNPG3T to the genus Brachybacterium while clearly distinguishing it from all currently described species within the genus. Accordingly, strain DNPG3T was proposed to represent a novel species, for which the name Brachybacterium netajii sp. nov. is suggested. The type strain was DNPG3T (= MTCC13125T).
More Related Videos
11:09Use of MALDI-TOF Mass Spectrometry and a Custom Database to Characterize Bacteria Indigenous to a Unique Cave Environment (Kartchner Caverns, AZ, USA)
Published on: January 2, 2015
06:34Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Related Concept Videos
Bacterial Phylum Bacteroidota
Bacterial Phylum Proteobacteria
Bacterial Phylum Actinobacteria
Deep Sea Microbial Ecology
Bacterial Phylum Planctomycetes
Applications of Molecular Taxonomy