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Updated: Apr 27, 2026

Isolation of Human Neutrophils from Whole Blood and Buffy Coats

Isolation of Human Neutrophils from Whole Blood and Buffy Coats

Published on: September 17, 2021

Eosinophil purification from peripheral blood.

Praveen Akuthota¹, Kelsey Capron, Peter F Weller

¹Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, KSB 23, 330 Brookline Avenue, Boston, 02215, MA, USA, pakuthot@bidmc.harvard.edu.

Methods in Molecular Biology (Clifton, N.J.)

|July 3, 2014

Summary

This summary is machine-generated.

Researchers developed a new method to isolate human eosinophils from blood. This technique aids in studying allergic inflammation and parasitic responses, crucial for understanding immune system functions.

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Area of Science:

Immunology
Cell Biology

Background:

Eosinophils are key granulocytes involved in allergic inflammation and parasitic responses.
They constitute 1-4% of circulating leukocytes in healthy humans.
Studying eosinophils is vital for understanding allergic mechanisms.

Purpose of the Study:

To describe a novel technique for isolating human eosinophils.
To facilitate ex vivo and in vitro research on eosinophil functions.

Main Methods:

Isolation of human eosinophils using negative selection.
Utilizing whole blood obtained via venipuncture.

Main Results:

A described technique for isolating human eosinophils from whole blood.

Conclusions:

The described negative selection method provides a valuable tool for eosinophil research.
This technique supports further investigation into allergic inflammation and parasitic responses.