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Updated: Apr 27, 2026
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Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung
Published on: November 16, 2021
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CD34+ eosinophil-lineage-committed cells in the mouse lung.
Apostolos Bossios1, Madeleine Rådinger
1Krefting Research Centre, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Methods in Molecular Biology (Clifton, N.J.)
|July 3, 2014
Summary
Eosinophil progenitor cells can proliferate outside the bone marrow and worsen chronic inflammation. This chapter details methods for detecting these cells in mouse lungs and assessing their proliferation.
Area of Science:
- Immunology
- Cell Biology
- Respiratory Medicine
Background:
- Eosinophil progenitor cells contribute to chronic inflammation through mediator production and cell recruitment.
- Extramedullary proliferation of eosinophil progenitors exacerbates inflammatory conditions.
Purpose of the Study:
- To provide methodologies for detecting eosinophil progenitor cells in the lung.
- To establish methods for monitoring the proliferation capacity of these cells in vivo.
Main Methods:
- Utilizing allergen-challenged mouse models.
- Implementing techniques for the detection and quantification of eosinophil progenitor cells in lung tissue.
- Assessing progenitor cell proliferation capacity.
Main Results:
- The study successfully outlines methods for identifying and evaluating eosinophil progenitor cells in the lung.
- The provided techniques allow for the monitoring of eosinophil progenitor cell proliferation in response to inflammatory stimuli.
Conclusions:
- The developed methods enable researchers to study the role of eosinophil progenitor cells in lung inflammation.
- Understanding eosinophil progenitor cell proliferation is crucial for developing targeted therapies for chronic inflammatory diseases.
