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Cell Lines

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A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...

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Related Experiment Video

Updated: Apr 27, 2026

Author Spotlight: Investigating the Pathophysiology of Eosinophilic Esophagitis

Author Spotlight: Investigating the Pathophysiology of Eosinophilic Esophagitis

Published on: May 10, 2024

Eosinophil cell lines.

Kenji Ishihara¹

¹Laboratory of Medical Science, Course for School Nurse Teacher, Faculty of Education, Ibaraki University, 2-1-1 Bunkyo, Mito, Ibaraki, 310-8512, Japan, ishihara@mx.ibaraki.ac.jp.

Methods in Molecular Biology (Clifton, N.J.)

|July 3, 2014

Summary

This summary is machine-generated.

Continuous histone acetylation drives eosinophil differentiation in HL-60 clone 15 and EoL-1 cells. Histone deacetylase inhibitors like sodium butyrate induce this process through sustained histone hyperacetylation, promoting gene transactivation.

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Area of Science:

Cell biology
Molecular biology
Epigenetics

Background:

Eosinophilic cell lines (HL-60 clone 15, EoL-1) are crucial models for studying eosinophil differentiation.
Histone acetylation plays a key role in regulating gene expression during cellular differentiation.

Purpose of the Study:

To elucidate the role of histone acetylation in eosinophil differentiation.
To describe methods for maintaining and differentiating HL-60 clone 15 and EoL-1 cells into eosinophils.

Main Methods:

Utilizing HL-60 clone 15 and EoL-1 cell lines.
Employing histone deacetylase inhibitors, specifically sodium butyrate.
Monitoring histone acetylation levels and gene transactivation.

Main Results:

Continuous histone acetylation, induced by sodium butyrate, promotes eosinophil differentiation.
Histone hyperacetylation leads to the transactivation of genes essential for eosinophil development.
Transient acetylation by trichostatin A does not induce eosinophilic differentiation.

Conclusions:

Sustained histone acetylation is critical for inducing eosinophil differentiation in specific cell lines.
Sodium butyrate effectively promotes eosinophil differentiation via continuous histone acetylation.
Understanding these mechanisms can inform research on eosinophil-related biological processes.