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Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis
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Identification of human eosinophils in whole blood by flow cytometry
Caroline Ethier1, Paige Lacy, Francis Davoine
1Pulmonary Research Group, University of Alberta, Edmonton, AB, Canada.
Methods in Molecular Biology (Clifton, N.J.)
|July 3, 2014
Abstract:
Identification of eosinophils in whole blood samples by flow cytometry is often problematic. There are usually only a low number and percentage of cells that may be detected, and it may be difficult to discriminate eosinophils from other granulocytes. Here, we propose a simple approach using the eosinophil's intrinsic autofluorescence properties, combined with detection of CCR3 expression, to reliably identify eosinophils in a mixed leukocyte population.
