Sensitive detection of platelet-derived growth factor through surface-enhanced Raman scattering

Area of Science:

• Nanotechnology
• Biochemistry
• Analytical Chemistry

Background:

• Platelet-derived growth factor (PDGF) is crucial in cellular processes.
• Sensitive detection of PDGF is vital for diagnosing various diseases.
• Existing detection methods often lack sensitivity and selectivity.

Purpose of the Study:

• To develop a highly sensitive and selective surface-enhanced Raman scattering (SERS) assay for PDGF detection.
• To utilize novel nanomaterials for enhanced signal generation and assay reproducibility.
• To demonstrate the assay's potential for analyzing PDGF in complex biological matrices like urine.

Main Methods:

• Conjugation of gold nanoparticles (Au NPs) with aptamer (Apt) and 4-mercaptobenzoic acid (MBA) for PDGF recognition and signal reporting.
• Utilizing gold pearl necklace nanomaterials (Au PNNs) to amplify the SERS signal of 4-MBA.
• Employing a specific binding mechanism between Apt and PDGF, followed by electrostatic interaction for aggregation with Au PNNs.

Main Results:

• Achieved high enhancement factors (up to 1.3 × 10^7) and reproducible SERS signals (RSD down to 15%).
• Demonstrated sensitive detection of PDGF BB down to 0.5 pM with good linearity over 1-50 pM.
• Successfully applied the assay for PDGF determination in urine samples, showcasing its practical applicability.

Conclusions:

• The developed SERS assay offers high sensitivity and selectivity for PDGF detection.
• The combination of Au NPs and Au PNNs provides a robust platform for ultrasensitive protein analysis.
• This assay holds significant potential for early disease diagnosis and monitoring through biological sample analysis.