Related Experiment Videos
HLA class II typing using oligonucleotide probes
D M Robinson1, S Holbeck, C Seyfried
1Virginia Mason Research Center, Seattle, WA 98101.
Genetic Epidemiology
|January 1, 1989
Abstract:
A DQ beta locus-specific oligonucleotide probe is used to identify DQ beta alleles present in six insulin-dependent diabetes mellitus (IDDM) multiplex families. Results of genotyping using this technique agree with those from cDNA probes and are often clearer. Allele-specific oligonucleotide probes for the DR beta locus are used to identify subtypes (Dw4 and Dw14) of the DR4 specificity in five IDDM multiplex families. This distinction cannot be made with available cDNA probes.