Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

3.1K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
3.1K
CRISPR01:59

CRISPR

46.3K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
46.3K
What is Genetic Engineering?00:49

What is Genetic Engineering?

70.4K
Overview
70.4K
CRISPR and crRNAs02:53

CRISPR and crRNAs

14.5K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
14.5K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

5.7K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
5.7K
Homologous Recombination02:31

Homologous Recombination

58.7K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
58.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genotoxicity profiling reveals distinct platform-and cell type-specific effects in therapeutic gene editing for genetic hyperinflammation.

Cell stem cell·2026
Same author

Engineering a compact high-fidelity Staphylococcus aureus Cas9 variant with broader targeting range and mechanistic insights into its activation.

Nature communications·2026
Same author

Monitoring biological effects of somatic cell genome editing.

Nature reviews. Genetics·2026
Same author

Sensitive and unbiased genome-wide profiling of base-editor-induced off-target activity using CHANGE-seq-BE.

Nature biotechnology·2026
Same author

Brain-wide Genome Editing via STEP-RNPs for Treatment of Angelman Syndrome.

bioRxiv : the preprint server for biology·2025
Same author

Measurement and clinical interpretation of CRISPR off-targets.

Nature genetics·2025
Same journal

Patient-derived organoids reveal ductal dysfunction and CFTR-modulator responses in chronic pancreatitis.

Cell stem cell·2026
Same journal

Lineage plasticity driven by GATA6 loss fuels colorectal cancer metastasis.

Cell stem cell·2026
Same journal

Quantitative molecular cartography of emergency myelopoiesis reveals conserved modules of hematopoietic activation.

Cell stem cell·2026
Same journal

Paired pre- and post-transplant human immunoprofiling identifies an IFN-γ-JAK1 axis limiting stem-cell-derived RPE engraftment.

Cell stem cell·2026
Same journal

ENPP1 blockade with a humanized monoclonal antibody enhances renal repair after acute kidney injury.

Cell stem cell·2026
Same journal

ZNF512B safeguards genome integrity at regulatory regions to repress the SASP and inflammation.

Cell stem cell·2026
See all related articles

Related Experiment Video

Updated: Apr 27, 2026

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

21.5K

What's changed with genome editing?

Shengdar Q Tsai1, J Keith Joung1

  • 1Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA 02129, USA; Department of Pathology, Harvard Medical School, Boston, MA 02115, USA.

Cell Stem Cell
|July 5, 2014
PubMed
Summary
This summary is machine-generated.

Engineered nucleases enable precise genome editing, but unintended genetic mutations remain a concern. Whole-genome sequencing confirms that targeted pluripotent stem cells can be generated with minimal off-target effects.

More Related Videos

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

35.9K
CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
07:49

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

Published on: May 30, 2025

2.4K

Related Experiment Videos

Last Updated: Apr 27, 2026

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

21.5K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

35.9K
CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
07:49

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

Published on: May 30, 2025

2.4K

Area of Science:

  • Molecular Biology
  • Genetics
  • Stem Cell Research

Background:

  • Engineered nucleases offer precise genome editing capabilities for generating specific genetic mutations.
  • The spectrum of unintended off-target mutations induced by these technologies requires thorough investigation.

Discussion:

  • High-coverage whole-genome sequencing was employed to assess the accuracy of targeted genome editing in pluripotent stem cells.
  • The study evaluated the feasibility of generating precisely edited stem cell clones while minimizing unintended genetic alterations.

Key Insights:

  • Targeted genome editing in pluripotent stem cells can achieve high precision.
  • Minimizing off-target lesions is crucial for the safe application of genome editing technologies.

Outlook:

  • Further research should focus on refining genome editing tools to enhance specificity.
  • The findings support the potential of genome editing for therapeutic applications in stem cell biology.