Protein encapsulated core-shell structured particles prepared by coaxial electrospraying: investigation on material
Maedeh Zamani1, Molamma P Prabhakaran2, Eng San Thian3
1Department of Mechanical Engineering, National University of Singapore, 2 Engineering Drive 3, 117576, Singapore; Center for Nanofibers and Nanotechnology, E3-05-14, Nanoscience and Nanotechnology Initiative, Faculty of Engineering, National University of Singapore, 2 Engineering Drive 3, 117576, Singapore.
Coaxial electrospraying efficiently creates biodegradable PLGA core-shell particles for drug delivery. This method enhances protein encapsulation and controlled release compared to emulsion electrospraying, showing promise for therapeutic applications.
Area of Science:
- Biomaterials Science
- Polymer Chemistry
- Drug Delivery Systems
Background:
- Biodegradable polymeric particles are crucial for controlled drug delivery.
- Electrospraying techniques offer methods for fabricating these particles.
- Coaxial electrospraying is explored for creating core-shell structures.
Purpose of the Study:
- To fabricate core-shell poly(lactic-co-glycolic acid) (PLGA) particles using coaxial electrospraying.
- To encapsulate bovine serum albumin (BSA) as a model protein within these particles.
- To compare coaxial electrospraying with emulsion electrospraying for particle fabrication and protein encapsulation.
Main Methods:
- Coaxial and emulsion electrospraying techniques were employed.
- Two different molecular weights of PLGA were used for encapsulation.
- Particle morphology, diameter, encapsulation efficiency, and in vitro release were analyzed.
- Fluorescence microscopy confirmed the core-shell structure.
Main Results:
- Coaxial electrospraying successfully produced core-shell PLGA particles with diameters ranging from 3.0 to 5.5 μm.
- Encapsulation efficiency was significantly higher with coaxial electrospraying (15.7-25.1% increase).
- Low molecular weight PLGA particles showed higher BSA release over 43 days compared to high molecular weight PLGA particles.
Conclusions:
- Coaxial electrospraying is a viable technique for fabricating protein-loaded biodegradable core-shell particles.
- This method offers improved encapsulation efficiency and controlled release profiles.
- The technique holds potential for delivering sensitive therapeutic agents like growth factors.
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