Related Experiment Video
Updated: Apr 27, 2026
12:02
Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
Published on: June 6, 2017
26.6K
Cannabinoid receptor activation inhibits cell cycle progression by modulating 14-3-3β
Hye-Won Jung1, Inae Park, Sungho Ghil
1Department of Life Science, Kyonggi University, Suwon, 443-760, Republic of Korea.
Cellular & Molecular Biology Letters
|July 9, 2014
Summary
Cannabinoids activate the 14-3-3β protein, leading to a delay in cell cycle progression at the G2/M phase. This discovery sheds light on the molecular mechanisms behind cannabinoid
Area of Science:
- Molecular Pharmacology
- Cell Biology
- Cannabinoid Research
Background:
- Cannabinoids exhibit diverse pharmacological effects, including anti-tumor, anti-inflammatory, and neuroprotective properties.
- Understanding the molecular pathways of cannabinoid action is crucial for therapeutic development.
Purpose of the Study:
- To elucidate the molecular mechanisms underlying cannabinoid pharmacological effects.
- To identify proteins interacting with the type 1 cannabinoid receptor (CB1R).
Main Methods:
- Yeast two-hybrid system screening of a mouse brain cDNA library.
- Affinity-binding assays to confirm protein interactions.
- Cell cycle analysis in HeLa cells using a double-thymidine block-and-release protocol.
Main Results:
- Identified 14-3-3β as a binding partner of CB1R.
- Cannabinoid treatment induced cell cycle arrest at the G2/M phase in HeLa cells.
- CB1R activation enhanced the interaction between 14-3-3β, Wee1, and Cdc25B, promoting Cdc2 phosphorylation.
Conclusions:
- Cannabinoids induce G2/M phase cell cycle delay through the activation of 14-3-3β.
- The interaction between CB1R, 14-3-3β, and cell cycle regulators (Wee1, Cdc25B) is a key mechanism.
- This finding provides novel insights into cannabinoid-mediated cell cycle regulation.
Related Concept Videos
02:34Inhibition of Cdk Activity
4.8K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.8K
02:34Inhibition of CDK Activity
4.3K
4.3K
01:23Negative Regulator Molecules
32.1K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
32.1K
01:27Molecular Factors Affecting Cell Division
3.9K
Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
3.9K
02:39Positive Regulator Molecules
5.3K
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
5.3K
01:45Positive Regulator Molecules
100.8K
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
100.8K