Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Animal Mitochondrial Genetics02:59

Animal Mitochondrial Genetics

7.8K
Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
7.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessment of the bacterial community of the human upper respiratory tract in patients affected by Covid-19.

Genetics and molecular biology·2026
Same author

The genome sequence of the açaí berry (<i>Euterpe oleracea</i> Mart.) and RNA-Seq analysis of the fruit ripening.

Genome·2026
Same author

The first complete mitogenome of Cynoscion acoupa (Lacepède, 1801), an vulnerable sciaenidae fish from Amazon coast: phylogenetic relationship and implications for conservation.

Anais da Academia Brasileira de Ciencias·2026
Same author

The mitogenome mutation repertoire affects progression of Parkinson's Disease.

Genetics and molecular biology·2026
Same author

Molecular analysis of mitochondrial complex I in the levodopa short duration response in Parkinson's disease.

Parkinsonism & related disorders·2026
Same author

Mitochondrial insertions/deletions (INDELs) burden in Parkinson's disease: an analysis from a Brazilian cohort.

Parkinsonism & related disorders·2026
Same journal

Hidden in the Pangenome? Machine Learning-Driven Discovery of Antimicrobial Potential in <i>Corynebacterium glutamicum</i>.

Bioinformatics and biology insights·2026
Same journal

<i>In silico</i> Design and Analysis of Engineered Proteins Containing Multi-Epitope and Immunodominant Domains Derived From <i>Rickettsia prowazekii A</i>ntigens.

Bioinformatics and biology insights·2026
Same journal

Chemoinformatic Approaches to Identify Bioactive Inhibitors Against Type I Dehydroquinase (DHQ1) Enzyme of Typhoidal <i>Salmonella</i>.

Bioinformatics and biology insights·2026
Same journal

Web-Based Graphical User Interface Design Integrating MATLAB Server for the Mathematical Model of Human Cardiovascular-Respiratory System.

Bioinformatics and biology insights·2026
Same journal

Listeria Genome Identification Using DNABERT Embedding With LightGBM and SHAP-Based Explainable Classification.

Bioinformatics and biology insights·2026
Same journal

A Novel Bioinformatics Pipeline and a Machine-Learning Approach for Antimicrobial Resistance Phenotypic Prediction.

Bioinformatics and biology insights·2026
See all related articles

Related Experiment Video

Updated: Apr 27, 2026

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA
12:35

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA

Published on: November 14, 2017

9.6K

A Protocol for mtGenome Analysis on Large Sample Numbers.

Igor G Hamoy1, André M Ribeiro-Dos-Santos2, Luiz Alvarez3

  • 1Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil. ; Universidade Federal Rural da Amazônia, Campus de Capanema, PA, Brazil.

Bioinformatics and Biology Insights
|July 9, 2014
PubMed
Summary
This summary is machine-generated.

This study presents an efficient protocol for sequencing the complete mitochondrial genome using the Ion PGM™ platform. This method enables high-quality, comprehensive analysis of mitochondrial DNA variability across numerous samples.

Keywords:
analysis protocolmitochondrial DNAnext-generation sequencingpolymorphismpopulation genetics

More Related Videos

Author Spotlight: High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution
10:47

Author Spotlight: High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution

Published on: May 5, 2023

4.1K
Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
07:24

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

Published on: February 10, 2023

2.0K

Related Experiment Videos

Last Updated: Apr 27, 2026

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA
12:35

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA

Published on: November 14, 2017

9.6K
Author Spotlight: High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution
10:47

Author Spotlight: High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution

Published on: May 5, 2023

4.1K
Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
07:24

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

Published on: February 10, 2023

2.0K

Area of Science:

  • Genetics
  • Genomics
  • Bioinformatics

Background:

  • Mitochondrial genome studies are crucial in population, forensic, and medical genetics.
  • Previous research often focused on limited, highly variable regions, missing total genomic variability.
  • High-throughput sequencing technologies now allow for more extensive and affordable mitochondrial genome analysis.

Purpose of the Study:

  • To develop and describe a protocol for sequencing and analyzing the complete mitochondrial genome.
  • To utilize the Ion PGM™ platform for this comprehensive genomic analysis.
  • To evaluate the efficiency and accuracy of the developed protocol.

Main Methods:

  • Sequencing of the complete mitochondrial genome using the Ion PGM™ platform.
  • Analysis of 12 samples with an average coverage of 1023× per sample.
  • Comparison of multiple variant callers to optimize protocol outcomes.

Main Results:

  • Achieved sequencing of approximately 210 Mbp of high-quality mitochondrial genome data.
  • Demonstrated the capability to process up to 120 samples per run without significant quality loss.
  • Identified an efficient and accurate protocol for full mitochondrial genome analysis.

Conclusions:

  • The developed protocol offers an efficient and accurate method for complete mitochondrial genome sequencing and analysis.
  • This protocol supports large-scale studies by enabling high-throughput processing of samples.
  • Facilitates deeper insights into mitochondrial DNA variability for various genetic applications.