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Cloning and characterization of araA, araB, and araD, the structural genes for L-arabinose utilization in Bacillus
I Sá-Nogueira1, H de Lencastre
1Laboratório de Genetica Molecular, Instituto Gulbenkian de Ciência, Oeiras, Portugal.
Abstract:
Two recombinant plasmids, pSNL1 and pSNL2, carrying structural genes for L-arabinose utilization were isolated from a Bacillus subtilis gene library. Both plasmids complemented araD mutations in a Rec- B. subtilis strain and in Escherichia coli. Moreover, pSNL1 also complemented araB mutations in both species and efficiently transformed araA Rec+ B. subtilis strains to Ara+. Detailed physical mapping of both plasmids in addition to transformation experiments involving defined restriction fragments from the pSNL1 insert unambiguously determined the gene order to be araD, araB, and araA, an order different from that found in E. coli.