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Updated: Apr 27, 2026

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Synthesis of Indoxyl-glycosides for Detection of Glycosidase Activities
Published on: May 27, 2015
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Fluoro-glycosyl acridinones are ultra-sensitive active site titrating agents for retaining β-glycosidases
Tianmeng Duo1, Ethan D Goddard-Borger, Stephen G Withers
1The Department of Chemistry, The University of British Columbia, 2036 Main Mall, Vancouver, BC, Canada V6T 1Z1. withers@chem.ubc.ca.
Abstract:
Novel fluorogenic 2-deoxy-2-fluoroglycosyl acridinone active site titrating reagents were synthesised and kinetic parameters determined for their inactivation of two retaining β-glucosidases, a β-galactosidase, a β-xylosidase and several cellulases. Fluorescence-monitored active site titration using this class of reagents reliably measured active enzyme concentrations down to 3 nM.

