Related Experiment Video
Updated: Aug 12, 2026

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
Published on: February 18, 2014
[An algorithm for calculating and analyzing the dissociation curves of human blood oxyhemoglobin standardized by 2
Abstract:
An algorithm of an "OXYHEM" programme intended for standardization of experimentally changed human blood oxyhemoglobin dissociation curves by two (H+, CO2) and three (H+, CO2, 2,3-DPG) allosteric ligands is presented. In the former case all the points of the curve are adjusted to the values of pH--7.4, pCO2--40 mmHg, while in the latter case--to values of pH--7.4, pCO2--40 mmHg and 2,3-DPG concentration 5 mmol per 1 of PBC. Every standardized curve makes it possible to determine P50, Hill's ratio, arteriovenous difference by O2 saturation and content. A comparative analysis of the parameters calculated presents a wide range of data reflecting the state of oxygen transport hemoglobin function in the studied blood.
More Related Videos
12:38Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction
Published on: February 15, 2018
09:24Synthesis, Hemoglobin Encapsulation and Biorthogonal PEGylation in Hierarchically Porous UiO-66 Nanoparticles for Oxygen Delivery Applications
Published on: May 8, 2026
Related Concept Videos
Gene Families
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
The Equilibrium Binding Constant and Binding Strength
Ligand Binding and Linkage
Cooperative Allosteric Transitions
Complexometric Titration: Ligands