Related Experiment Video

Updated: Apr 27, 2026

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer
09:40

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

3.2K

Downregulated miR-646 in clear cell renal carcinoma correlated with tumour metastasis by targeting the nin one

W Li1, M Liu1, Y Feng2

  • 1Department of Urology, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, China.

British Journal of Cancer
|July 11, 2014
PubMed
Abstract

Insights

MicroRNA-646 (miR-646) downregulation in clear cell renal cell carcinoma (ccRCC) promotes metastasis by targeting Nin one binding protein (NOB1) via the MAPK pathway. Restoring miR-646 may inhibit ccRCC development.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Nin one binding protein (NOB1) is a potential oncogene in glioma.
  • MicroRNA-646 (miR-646) is crucial for growth and development.
  • The roles of NOB1 and miR-646 in renal cell carcinoma (RCC) tumorigenesis are unknown.

Purpose of the Study:

  • Investigate the molecular mechanisms of NOB1 in RCC.
  • Determine the correlation between NOB1 and miR-646 in ccRCC.
  • Assess the potential of miR-646 as a therapeutic target.

Main Methods:

  • Bioinformatic analysis to identify miRNA targeting NOB1.
  • Quantitative real-time PCR to measure NOB1 and miR-646 expression in ccRCC tissues.
  • In vitro proliferation assays (colony formation) and in vivo xenograft models.
  • Correlation analysis between miR-646, NOB1, tumor features, and patient survival.

Main Results:

  • miR-646 negatively regulates NOB1, inhibiting renal cancer cell proliferation and migration.
  • NOB1 is upregulated, while miR-646 is downregulated in ccRCC, particularly in metastatic cases.
  • miR-646 expression is inversely correlated with NOB1 and predicts a higher risk of metastasis.
  • NOB1 suppression affects MAPK pathway signaling.

Conclusions:

  • Downregulated miR-646 in ccRCC is linked to metastasis via NOB1 targeting and the MAPK pathway.
  • miR-646 and NOB1 are significant players in ccRCC development and progression.
  • miR-646 warrants further investigation as a potential biomarker and therapeutic target for ccRCC.

More Related Videos

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization06:01

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization

Published on: June 7, 2016

6.3K
CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

2.5K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
20.9K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
7.5K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
3.7K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.0K