Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sensibility assay for topical agents. A new method.

A S Lorenti1, J M Bensadon-Carbonell, F Benaim

  • 1Bacteriology Laboratory, Burn Foundation, Buenos Aires, Argentina.

The Journal of Burn Care & Rehabilitation
|May 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antiseptic-impregnated central venous catheters: their evaluation in burn patients.

Annals of burns and fire disasters·2011
Same author

Peri-Operative Bacteraemia in Burn Patients. What Does it Mean?

Annals of burns and fire disasters·2011
Same author

[Hepatic stem cells].

Medicina·2001
Same author

Massage in hypertrophic scars.

The Journal of burn care & rehabilitation·1999
Same author

Development in the treatment of burns in South America during the last decades.

Burns : journal of the International Society for Burn Injuries·1999
Same author

Pulsed electromagnetic fields in experimental cutaneous wound healing in rats.

The Journal of burn care & rehabilitation·1996

A new laboratory method efficiently tests topical agent sensitivity in clinical bacterial strains. This simple, economical, and reproducible method determines the minimal inhibitory concentration for effective antimicrobial susceptibility testing.

Area of Science:

  • Microbiology
  • Laboratory Medicine
  • Pharmacology

Background:

  • Accurate antimicrobial susceptibility testing is crucial for effective treatment of bacterial infections.
  • Existing methods for testing topical agents may be complex or costly.
  • There is a need for a simple, reproducible laboratory method to assess bacterial sensitivity to topical agents.

Purpose of the Study:

  • To introduce and validate a novel laboratory method for determining the sensitivity of clinically isolated bacterial strains to topical agents.
  • To establish a straightforward and economical approach for calculating the minimal inhibitory concentration (MIC) of topical antimicrobial formulations.

Main Methods:

  • A whole cream dilution method in a solid agar medium was developed.
  • Creams were suspended in sterile water at 45°C, mixed with agar at 45°C, and plated.

Related Experiment Videos

  • Twenty-five bacterial strains were inoculated using a Multinoculator and incubated for 18 hours at 37°C.

    • Main Results:

    • The method allows for the determination of bacterial growth or inhibition after incubation.
    • The minimal inhibitory concentration (MIC) is identified as the lowest concentration of the topical agent that prevents bacterial growth.
    • The developed method is characterized as simple, economical, and reproducible.

    Conclusions:

    • The presented laboratory method offers a practical and efficient way to test the sensibility of clinical bacterial isolates to topical agents.
    • This technique provides a reliable means for determining MIC, aiding in the selection of appropriate topical antimicrobial therapies.
    • The simplicity, cost-effectiveness, and reproducibility of this method make it suitable for routine laboratory use.