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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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Related Experiment Video

Updated: Apr 27, 2026

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

Published on: April 11, 2019

Analyzing the first drafts of the human proteome.

Iakes Ezkurdia, Jesús Vázquez, Alfonso Valencia¹

¹‡Spanish National Cancer Research Centre (CNIO), Melchor Fernandez Almagro, 3, Madrid 28029, Spain.

Journal of Proteome Research

|July 12, 2014

Summary

Two recent human proteome studies likely overestimated their findings. A quality check using olfactory receptors suggests caution when interpreting their protein-coding gene identification data.

Keywords:

Nature human proteome olfactory receptors protein coding genes proteomics

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Last Updated: Apr 27, 2026

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Area of Science:

Proteomics
Genomics
Molecular Biology

Background:

Two large-scale human proteome studies were published, presented as significant advancements.
These studies aimed to create draft maps of the human proteome.
The reported number of identified protein-coding genes exceeded expectations from established efforts like the Human Proteome Project.

Discussion:

A quality control assessment focused on olfactory receptors, a gene family not specifically targeted by the studies.
Unexpectedly high peptide evidence for olfactory receptors was found in both studies, despite lack of nasal tissue analysis.
This suggests a potential overestimation of identified protein-coding genes in the analyzed datasets.

Key Insights:

The studies likely overestimate the number of protein-coding genes identified.
Olfactory receptor data indicates potential issues with the scale and specificity of the proteomics experiments.
Findings challenge the initial portrayal of these studies as definitive human proteome maps.

Outlook:

Further validation and careful re-analysis of the experimental data are recommended.
The scientific community should exercise caution when utilizing the findings from these two proteomics studies.
This critique highlights the importance of rigorous quality control in large-scale omics research.