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Flow Cytometric Characterization of Murine B Cell Development
Published on: January 22, 2021
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Purification and immunophenotypic characterization of murine B10 B cells
1Institute of Biology and Medical Sciences, Soochow University, 199 Ren-Ai Road, Suzhou Industrial Park, Suzhou, 215123, Jiangsu Province, China.
Methods in Molecular Biology (Clifton, N.J.)
|July 13, 2014
Summary
Regulatory B10 cells are rare IL-10 secreting B cells that control immune responses. This study details methods for identifying and purifying these crucial CD1d(hi)CD5(+) B10 cells in mice.
Area of Science:
- Immunology
- Cell Biology
Background:
- Regulatory B10 cells are a rare subset of B cells in mice, characterized by CD1d(hi)CD5 expression.
- These cells secrete Interleukin-10 (IL-10) and play a critical role in modulating T cell-mediated immune responses.
- Their regulatory functions are vital in managing inflammatory and autoimmune diseases.
Purpose of the Study:
- To describe established methods for the identification of regulatory B10 cells.
- To outline techniques for the purification of CD1d(hi)CD5(+) B10 cells.
Main Methods:
- In vitro stimulation of B cells with mitogens and chemical stimulants to induce IL-10 production.
- Flow cytometry for identification using CD1d(hi)CD5(+) markers.
- Cell sorting techniques for purification of the target B cell population.
Main Results:
- Regulatory B10 cells are identified as a CD1d(hi)CD5(+) IL-10-secreting subset.
- These cells constitute a small fraction (1-2%) of splenic B cells.
- Their induction of IL-10 secretion occurs after specific in vitro stimulation.
Conclusions:
- Effective methods for identifying and purifying regulatory B10 cells are crucial for further research.
- Understanding these cells aids in developing strategies for autoimmune and inflammatory disease treatment.
- The antigen-specific and IL-10-dependent mechanisms of B10 cells highlight their immunomodulatory importance.
