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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
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Sensitive detection of microRNA by chronocoulometry and rolling circle amplification on a gold electrode
Bo Yao1, Yichen Liu, Miyuki Tabata
1Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310058, China. yaobo08@zju.edu.cn.
Chemical Communications (Cambridge, England)
|July 15, 2014
Abstract:
We developed a quantitative detection scheme for nucleic acids, combining solid-phase rolling circle amplification and chronocoulometry (RCA-CC). A gold electrode was directly formed on a polystyrene substrate as a cost-effective and flexible biosensor for sensitive detection of microRNA (mir-143) in blood samples.
