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Exploring luminescence-based temperature sensing using protein-passivated gold nanoclusters.

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Protein-protected gold nanoclusters (AuNCs) show hysteresis when heated and cooled. Strategies like sol-gel coating eliminate this, making AuNCs viable for optical temperature monitoring.

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Area of Science:

  • Nanotechnology
  • Biomaterials Science
  • Analytical Chemistry

Background:

  • Optically monitoring aqueous-phase temperature is crucial for various scientific applications.
  • Protein-protected gold nanoclusters (AuNCs) offer potential as optical temperature sensors due to their unique optical properties.
  • Understanding the stability and limitations of these bio-passivated nanomaterials under thermal stress is essential for their practical application.

Purpose of the Study:

  • To investigate the analytical performance and limitations of protein-protected gold nanoclusters (AuNCs) for optical temperature monitoring in aqueous solutions.
  • To identify and characterize undesirable behaviors, such as hysteresis, in AuNC-based thermometers during thermal cycling.
  • To explore methods for mitigating these limitations and establishing the viability of AuNCs as reliable nanothermometers.

Main Methods:

  • Synthesis of protein-protected gold nanoclusters (AuNCs).
  • Optical characterization of AuNCs under varying temperatures.
  • Thermal cycling experiments to assess hysteresis.
  • Application of mitigation strategies including sol-gel coating and thermal denaturation of the protein template.

Main Results:

  • Protein-protected AuNCs exhibit significant hysteresis upon thermal cycling, a phenomenon not previously reported.
  • This hysteresis impacts the accuracy and reliability of AuNCs as temperature sensors.
  • Sol-gel coating and thermal denaturation of the biomolecular template effectively eliminate the observed hysteresis.

Conclusions:

  • Protein-templated AuNCs can be engineered into reliable optical nanothermometers.
  • Mitigation of thermal hysteresis is key to unlocking the potential of bio-passivated AuNCs for precise temperature sensing.
  • The findings pave the way for the development of advanced, protein-templated nanomaterial-based sensors.