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Updated: Apr 27, 2026
Optimized Protocols for Mycobacterium leprae Strain Management: Frozen Stock Preservation and Maintenance in Athymic Nude Mice
Published on: March 23, 2014
Semi-automated protocol for purification of Mycobacterium leprae from tissues using the gentleMACS™ Octo Dissociator
Diana L Williams1, Linda B Adams1, Ramanuj Lahiri1
1Department of Health and Human Services, Health Resource Services Administration, Health Systems Bureau, National Hansen's Disease Programs, Laboratory Research Branch, SVM-LSU, RM 3517W, Skip Bertman Dr., Baton Rouge, LA 70803, USA.
Abstract:
Mycobacterium leprae, etiologic agent of leprosy, is propagated in athymic nude mouse footpads (FPs). The current purification protocol is tedious and physically demanding. A simpler, semi-automated protocol was developed using gentleMACS™ Octo Dissociator. The gentleMACS protocol provided a very effective means for purification of highly viable M. leprae from tissue.
Insights
A new semi-automated method simplifies Mycobacterium leprae purification from mouse models. This gentleMACS protocol offers an effective alternative for researchers studying leprosy.
Area of Science:
- Microbiology
- Immunology
- Infectious Diseases
Background:
- Mycobacterium leprae is the causative agent of leprosy.
- Propagation in athymic nude mouse footpads (FPs) is a common method for M. leprae culture.
- Current M. leprae purification protocols are labor-intensive and physically demanding.
Purpose of the Study:
- To develop a simpler, semi-automated protocol for M. leprae purification.
- To evaluate the efficacy of the gentleMACS™ Octo Dissociator for M. leprae isolation.
Main Methods:
- A semi-automated protocol using the gentleMACS™ Octo Dissociator was developed.
- The protocol facilitates the purification of M. leprae from infected mouse tissues.
Main Results:
- The gentleMACS protocol provides a highly effective means for M. leprae purification.
- The method simplifies the previously tedious and physically demanding purification process.
- Purified M. leprae maintained high viability.
Conclusions:
- The gentleMACS protocol is a viable and efficient alternative for M. leprae purification.
- This method reduces the physical burden on researchers.
- The protocol supports further research into leprosy pathogenesis and treatment.
