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Related Concept Videos

Bioreactor Design and Operational System01:29

Bioreactor Design and Operational System

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Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
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Bioreactor Controls-I01:28

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Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly...
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Bioreactor Controls-II01:18

Bioreactor Controls-II

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In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the...
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Microbial Biosensors01:17

Microbial Biosensors

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Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
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A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria
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Novel Hydrogen Bioreactor and Detection Apparatus.

Joseph A Rollin1,2,3, Xinhao Ye1, Julia Martin Del Campo1

  • 1Biological Systems Engineering Department, Virginia Tech, 304 Seitz Hall, Blacksburg, VA, 24061, USA.

Advances in Biochemical Engineering/Biotechnology
|July 16, 2014
PubMed
Summary

This study presents a novel bioreactor for efficient in vitro hydrogen generation using enzymes. The system enables high-rate biohydrogen production from carbohydrates, offering a sustainable fuel alternative.

Keywords:
BiohydrogenContinuous hydrogen detectionHydrogen bioreactorIn vitro synthetic biosystem for biomanufacturingSynthetic pathway biotransformation

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Area of Science:

  • Biotechnology
  • Renewable Energy
  • Chemical Engineering

Background:

  • Hydrogen is a key commodity chemical with potential as a future transportation fuel.
  • Enzymatic biotransformation (SyPaB) allows hydrogen storage in carbohydrates.
  • Biohydrogen production offers solutions for distributed energy, reduced CO2, and cost-effectiveness.

Purpose of the Study:

  • To introduce a novel bioreactor for enzymatic hydrogen generation.
  • To develop an alternative calibration method for hydrogen sensors.
  • To demonstrate high-rate biohydrogen production from carbohydrates.

Main Methods:

  • Design and implementation of a novel bioreactor for oxygen-free enzymatic hydrogen generation.
  • Development of a flow calibration method for continuous hydrogen sensors.
  • Enzymatic conversion of glucose and glucose 6-phosphate to hydrogen.

Main Results:

  • The novel bioreactor successfully maintained an oxygen-free environment for hydrogen generation.
  • A flow calibration method was introduced as an alternative to electrolysis.
  • High hydrogen production rates were achieved, including 157 mmol/L/h from glucose 6-phosphate at 60 °C.

Conclusions:

  • The developed bioreactor and calibration method are effective for in vitro biohydrogen generation.
  • Enzymatic conversion of carbohydrates offers a promising route for sustainable hydrogen fuel production.
  • The system demonstrates potential for efficient, distributed bioenergy applications.