Related Experiment Video
Updated: Apr 27, 2026
11:43
Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors
Published on: May 31, 2012
169.5K
[Principles of antisepsis, disinfection and sterilization]
María-Jesús Hernández-Navarrete1, José-Miguel Celorrio-Pascual1, Carlos Lapresta Moros1
1Servicio de Medicina Preventiva y Salud Pública, Hospital Universitario Miguel Servet, Zaragoza, España.
Enfermedades Infecciosas Y Microbiologia Clinica
|July 16, 2014
Summary
This review covers infection prevention and control, focusing on antisepsis and disinfection using biocides. It highlights concerns about antimicrobial resistance linked to these chemicals and advances in sterilization techniques.
Area of Science:
- Infection Prevention and Control
- Microbiology
- Public Health
Background:
- Infection prevention and control are crucial for healthcare settings.
- Antisepsis and disinfection are key strategies to eliminate pathogens.
- The use of biocides raises concerns regarding antimicrobial resistance.
Purpose of the Study:
- To review the fundamental concepts of infection prevention and control.
- To discuss the principles and applications of antisepsis and disinfection.
- To highlight the implications of biocide use on antimicrobial resistance.
Main Methods:
- Review of scientific literature on infection control principles.
- Analysis of antisepsis and disinfection techniques.
- Discussion of biocide mechanisms and resistance concerns.
Main Results:
- Antisepsis and disinfection require prior environmental cleanup.
- Biocides used for disinfection share similarities with antibiotics, posing resistance risks.
- Sterilization methods include physical techniques like autoclaving and advancements in low-temperature methods.
Conclusions:
- Effective infection control relies on proper antisepsis, disinfection, and sterilization.
- Careful consideration of biocide use is necessary to mitigate antimicrobial resistance.
- Technological advancements are improving sterilization efficiency and safety.
Related Concept Videos
01:30Cleaning, Sterilization, and Disinfection
11.5K
Cleaning, disinfection, and sterilization are the methods that help to break the infection chain and prevent disease.
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
11.5K
01:23Hand hygiene
5.2K
Asepsis is the practice of preventing or breaking the chain of infection. The nurse employs aseptic techniques to prevent the spread of microorganisms and reduce the risk of diseases. Hand hygiene is the cornerstone of aseptic techniques and is classified into medical and surgical asepsis. Medical asepsis includes hand hygiene and the use of gloves. Surgical asepsis, or the sterile technique, refers to practices that render and keep objects and areas free of microorganisms.
Hand washing...
Hand washing...
5.2K
01:28Asepsis
3.7K
The condition of being free from disease-causing living pathogens is asepsis. Aseptic techniques include a set of standard practices to achieve asepsis. An example is the regular environmental cleaning of all parts of the healthcare facility and hand hygiene at home before preparing or eating food. Medical and surgical asepsis in healthcare practice protects patients from harmful pathogens, minimizes the risk of contamination of susceptible sites, and reduces the risk of infection transmission.
3.7K
01:29Methods of Sterilization I: Physical Methods
25.2K
As used in a healthcare facility, sterilization destroys all microorganisms through physical or chemical methods. The physical method includes steam, dry heat, boiling water, and radiation.
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
25.2K
01:30Methods of Sterilization II: Chemical Methods
9.5K
In healthcare, the chemical method of sterilization uses chemical sterilants to treat surgical instruments and medical supplies to help prevent the transmission of infectious pathogens to patients. Due to heat sensitivity, most medical supplies and equipment should not be exposed to high temperatures. These parts include rubber, plastic, glass, and other similar elements.
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
9.5K
01:29Methods for Controlling Microbial Growth
2.7K
Microbial growth control refers to various methods employed to inhibit, reduce, or eliminate microorganisms to ensure safety and hygiene across different settings. These methods are categorized based on the target environment and the level of microbial control required.Biocides are versatile agents designed to control microorganisms by either inhibiting their growth or outright killing them. These agents work through various physical, chemical, mechanical, or biological mechanisms. The...
2.7K