Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Apr 27, 2026

Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy

Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy

Published on: August 22, 2018

Phantoms for diffuse optical imaging based on totally absorbing objects, part 2: experimental implementation.

Fabrizio Martelli¹, Paola Di Ninni¹, Giovanni Zaccanti¹

¹Dipartimento di Fisica e Astronomia dell'Università degli Studi di Firenze, Via G. Sansone 1, 50019 Sesto Fiorentino, Firenze, Italy.

Journal of Biomedical Optics

|July 16, 2014

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Optical characterization of turbid media in a cuvette geometry with a calibrated lookup table.

Applied optics·2026

Same author

Functional dual-slope frequency-domain near-infrared spectroscopy data interpreted with two- and three-layer models.

Biophotonics discovery·2026

Same author

Noninvasive measurements of skeletal muscle hemodynamics using frequency-domain near-infrared spectroscopy: contributions from adipose and bone tissues.

Biophotonics discovery·2026

Same author

Phantom for standardization in functional near-infrared spectroscopy, part 2: optical properties and Monte Carlo simulations.

Neurophotonics·2026

Same author

In-vivo optical properties spectra across five body locations on ten subjects using time-domain diffuse optics.

Scientific data·2026

Same author

Ocular diagnostics and occipital neurovascular coupling in ocular hypertension and open angle glaucoma.

Frontiers in neuroscience·2025

Same journal

Segmentation-guided photon pooling enables robust single-cell analysis and fast fluorescence lifetime imaging microscopy.

Journal of biomedical optics·2026

Same journal

Method of spatial scanning of modulated laser radiation for outline imaging of interphalangeal joints.

Journal of biomedical optics·2026

Same journal

Multimodal optical imaging for the assessment of the teratogenic effects of ethanol on zebrafish development.

Journal of biomedical optics·2026

Same journal

Fluorescence properties of collagen types I-V: a comprehensive study of spectral and lifetime characteristics.

Journal of biomedical optics·2026

Same journal

Spectral dependence of lipofuscin fluorescence lifetimes revealed by FLIM with a superconducting nanowire single-photon detector.

Journal of biomedical optics·2026

Same journal

Building the future of biophotonics through experiential education and seasonal schools.

Journal of biomedical optics·2026

See all related articles

This summary is machine-generated.

This study introduces a new phantom for diffuse optical imaging using totally absorbing objects. This reliable and accurate phantom effectively mimics absorption changes for improved imaging applications.

Area of Science:

Biomedical Optics
Medical Imaging Technology
Optical Physics

Background:

Diffuse optical imaging (DOI) is a non-invasive technique used for tissue analysis.
Accurate phantoms are crucial for validating DOI methods and understanding light propagation in tissues.
Previous theoretical work established the basis for using totally absorbing objects in phantoms.

Purpose of the Study:

To experimentally implement and validate a novel phantom for diffuse optical imaging.
To utilize totally absorbing objects within the phantom to simulate absorption perturbations.
To assess the reliability, accuracy, and robustness of the developed phantom.

Main Methods:

Constructed a phantom using black polyvinyl chloride (PVC) cylinders as totally absorbing objects.

More Related Videos

Author Spotlight: A Stable Phantom Material for Optical and Acoustic Imaging

Author Spotlight: A Stable Phantom Material for Optical and Acoustic Imaging

Published on: June 16, 2023

Fabrication and Characterization of Optical Tissue Phantoms Containing Macrostructure

Fabrication and Characterization of Optical Tissue Phantoms Containing Macrostructure

Published on: February 12, 2018

Related Experiment Videos

Last Updated: Apr 27, 2026

Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy

Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy

Published on: August 22, 2018

Author Spotlight: A Stable Phantom Material for Optical and Acoustic Imaging

Author Spotlight: A Stable Phantom Material for Optical and Acoustic Imaging

Published on: June 16, 2023

Fabrication and Characterization of Optical Tissue Phantoms Containing Macrostructure

Fabrication and Characterization of Optical Tissue Phantoms Containing Macrostructure

Published on: February 12, 2018

Employed a scattering medium of intralipid-20% and India ink as the absorber.

Validated the phantom using time-domain measurements and Monte Carlo simulations.

Main Results:

Demonstrated the reliability, accuracy, and robustness of the phantom in mimicking absorption perturbations.
Confirmed that the phantom can accurately represent typical absorbing features encountered in diffuse optical imaging.
Showcased the phantom's versatility in generating various absorption perturbations by adjusting inclusion volume and position.

Conclusions:

The experimentally validated phantom provides a reliable tool for diffuse optical imaging research.
The use of totally absorbing objects offers a flexible method for simulating diverse absorption scenarios.
This phantom technology advances the development and application of diffuse optical imaging techniques.