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Femtogram detection of horseradish peroxidase by a common desktop scanner
Shahila Parween1, Pradip Nahar1
1Department of Chemical and Systems Biology, CSIR-Institute of Genomics and Integrative Biology, Mall Road, New Delhi 110 007, India; Academy of Scientific and Innovative Research, Anushandhan Bhavan, 2, Rafi Marg, New Delhi 110001, India.
Abstract:
We report an image-based detection of horseradish peroxidase (HRP) by different color spaces. The results show excellent correlation between color saturation and absorbance (Pearson correlation coefficient; 0.9868) with respect to HRP. The present method can detect 185 and 46.45 fg/ml of HRP using o-phenylenediamine dihydrochloride and 3,3',5,5'-tetramethylbenzidine as chromogenic substrates respectively.
