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Related Experiment Video

Updated: Apr 27, 2026

Genome-wide Mapping of Drug-DNA Interactions in Cells with COSMIC Crosslinking of Small Molecules to Isolate Chromatin

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Published on: January 20, 2016

Sequence-specific DNA interactions with calixarene-based langmuir monolayers.

Vanessa Rullaud¹, Negar Moridi, Patrick Shahgaldian

¹Institute of Chemistry and Bioanalytics, University of Applied Sciences and Arts Northwestern Switzerland , Gründenstrasse 40, CH-4132 Muttenz, Switzerland.

Langmuir : the ACS Journal of Surfaces and Colloids

|July 17, 2014

Summary

Amphiphilic calixarene monolayers interact differently with DNA sequences. Poly(AT) DNA caused greater monolayer expansion than poly(GC) or random DNA, indicating sequence-dependent interactions.

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Area of Science:

Supramolecular Chemistry
Materials Science
Biophysics

Background:

Amphiphilic calixarenes self-assemble into Langmuir monolayers.
Understanding interactions between synthetic molecules and DNA is crucial for nanotechnology and biomedicine.

Purpose of the Study:

Investigate the interactions between a specific amphiphilic calixarene (p-guanidino-dodecyloxy-calix[4]arene) and short double-stranded DNA.
Determine if DNA sequence composition influences these interactions.

Main Methods:

Langmuir monolayer formation and compression using surface pressure-area (π-A) isotherms.
Surface ellipsometry to measure monolayer thickness and structure.
Brewster angle microscopy (BAM) for in-situ visualization of monolayer morphology.

Main Results:

The calixarene monolayer expanded upon interaction with DNA, with significant sequence-dependent differences observed.
Poly(AT) DNA induced a more pronounced monolayer expansion compared to poly(GC) and random DNA sequences.
Surface ellipsometry and BAM revealed differences in monolayer thickness and structure when formed on DNA subphases versus pure water, varying with DNA composition.

Conclusions:

The amphiphilic calixarene monolayer exhibits sequence-specific interactions with double-stranded DNA.
DNA base pair composition (AT vs. GC content) significantly modulates the self-assembly and structural properties of the calixarene monolayer.
These findings highlight the potential for designing sequence-selective molecular recognition systems.