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Related Concept Videos

Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

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The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
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Overview of Electron Microscopy01:25

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The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
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Transmission Electron Microscopy01:15

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In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
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Electron Microscope Tomography and Single-particle Reconstruction01:07

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
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Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

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Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
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Phase Contrast and Differential Interference Contrast Microscopy01:26

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Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Related Experiment Video

Updated: Apr 26, 2026

Correlative Light Electron Microscopy CLEM for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells
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Correlative video-light-electron microscopy: development, impact and perspectives.

Riccardo Rizzo1, Seetharaman Parashuraman, Alberto Luini

  • 1Institute of Protein Biochemistry, Consiglio Nazionale Delle Ricerche (CNR-IBP), Via P. Castellino 111, 80131, Naples, Italy.

Histochemistry and Cell Biology
|July 18, 2014
PubMed
Summary
This summary is machine-generated.

Correlative light-electron microscopy (CLEM) combines live-cell imaging with high-resolution electron microscopy. This powerful technique overcomes the resolution limits of light microscopy, revealing cellular context for dynamic structures.

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Area of Science:

  • Cell Biology
  • Microscopy Techniques
  • Developmental Biology

Background:

  • Green fluorescent protein (GFP)-based video microscopy offers insights into live-cell dynamics.
  • A key limitation is the insufficient resolution of light microscopy for many cellular structures.

Purpose of the Study:

  • To introduce and discuss correlative video-light-electron microscopy (CLEM) as a solution to overcome resolution limitations.
  • To explore the potential, limitations, and future perspectives of CLEM in biological research.

Main Methods:

  • CLEM integrates GFP-based video microscopy with electron microscopy.
  • Ancillary techniques include proper fixation, hybrid labeling, and retracing for correlative imaging.

Main Results:

  • CLEM provides high resolution and essential cellular context for dynamic fluorescent structures.
  • This integrated approach significantly enhances the analytical power of video microscopy.

Conclusions:

  • CLEM multiplies the capabilities of video microscopy, offering profound insights into cellular processes.
  • Correlative approaches are impactful in cell and developmental biology, integrating diverse imaging modalities.