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The lncRNA PCAT29 inhibits oncogenic phenotypes in prostate cancer

Rohit Malik1, Lalit Patel1, John R Prensner1

  • 1Michigan Center for Translational Pathology, University of Michigan, Ann Arbor, Michigan. Department of Pathology, University of Michigan, Ann Arbor, Michigan.

Molecular Cancer Research : MCR
|July 18, 2014
PubMed
Abstract

Insights

Prostate cancer-associated transcript 29 (PCAT29) is a novel long noncoding RNA (lncRNA) that acts as a tumor suppressor. Low PCAT29 expression indicates a higher risk of prostate cancer recurrence.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long noncoding RNAs (lncRNAs) are increasingly implicated in human cancer development and progression.
  • The specific roles and interactions of lncRNAs with established oncogenic or tumor-suppressive pathways remain underexplored.

Purpose of the Study:

  • To characterize the novel lncRNA, prostate cancer-associated transcript 29 (PCAT29).
  • To investigate the relationship between PCAT29 and the androgen receptor signaling pathway in prostate cancer.

Main Methods:

  • Prostate cancer xenograft models and chick chorioallantoic membrane assays were utilized.
  • PCAT29 expression levels were manipulated (knockdown and overexpression) to assess functional effects.
  • Analysis of PCAT29 expression in patient specimens was correlated with clinical outcomes.

Main Results:

  • PCAT29 expression is suppressed by dihydrotestosterone (DHT) and upregulated following castration therapy.
  • PCAT29 knockdown enhanced prostate cancer cell proliferation and migration.
  • PCAT29 overexpression suppressed tumor growth and metastasis in preclinical models.
  • Low PCAT29 expression in patient samples correlated with poor prognosis.

Conclusions:

  • PCAT29 is identified as an androgen receptor-repressed tumor suppressor lncRNA in prostate cancer.
  • Loss of PCAT29 may serve as a biomarker for identifying patients at increased risk of recurrence.

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