Sorting drops and cells with acoustics: acoustic microfluidic fluorescence-activated cell sorter

Area of Science:

• Biotechnology
• Microfluidics
• Cell Sorting

Background:

• Traditional fluorescence-activated cell sorting (FACS) and droplet sorting (FADS) have limitations in speed and versatility.
• There is a need for high-throughput cell sorting methods compatible with various sample types.

Purpose of the Study:

• To develop and demonstrate a versatile microfluidic cell sorter utilizing acoustic actuation.
• To achieve ultra-high sorting rates for both droplets and individual cells.

Main Methods:

• Development of a microfluidic device employing acoustic waves for actuation.
• Sorting of aqueous droplets at kHz rates into multiple channels.
• Direct sorting of fluorescently labeled mouse melanoma cells in a single-phase fluid.

Main Results:

• Demonstrated ultra-high sorting rates for droplets (several kHz).
• Successfully sorted individual cells without prior encapsulation.
• Showcased compatibility with standard cell sorting cytometers.

Conclusions:

• The acoustic microfluidic cell sorter offers a versatile and high-throughput solution for cell and droplet sorting.
• This technology expands the capabilities of traditional FACS and FADS, enabling sophisticated applications.