Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calculated bicarbonate or total carbon dioxide?

T D O'Leary1, S R Langton

  • 1Department of Biochemistry, Repatriation General Hospital, Heidelberg, Victoria, Australia.

Clinical Chemistry
|August 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spontaneous Complete Uterine Rupture in a Nonlaboring, Early Third-trimester Uterus: Missed Diagnosis by Ultrasound.

AJP reports·2013
Same author

Direct laparoscopic venous sampling to diagnose a small Sertoli-Leydig tumor.

Gynecologic oncology·2003
Same author

Sulindac to prevent recurrent preterm labor: a randomized controlled trial.

Obstetrics and gynecology·2001
Same author

The mutual influence of gaze and head orientation in the analysis of social attention direction.

The Quarterly journal of experimental psychology. A, Human experimental psychology·2000
Same author

You must see the point: automatic processing of cues to the direction of social attention.

Journal of experimental psychology. Human perception and performance·2000
Same author

Stability of ceftriaxone sodium in polypropylene syringes at -20, 4, and 20 degrees C.

American journal of health-system pharmacy : AJHP : official journal of the American Society of Health-System Pharmacists·1996
Same journal

From Pooled Cohorts to PREVENT: A Perspective for Clinical Laboratorians.

Clinical chemistry·2026
Same journal

Interlaboratory Comparison of a Glucagon and Oxyntomodulin Immuno-LC-MS/MS Assay: Implications for Diabetes Research.

Clinical chemistry·2026
Same journal

Comparison of Information-Dependent Acquisition and Sequential Window Acquisition of All Theoretical Mass Spectra for Untargeted Drug Testing on a Linear Ion Trap-Pulsing Quadrupole-Time of Flight Mass Spectrometer.

Clinical chemistry·2026
Same journal

Patterns of One-Year Change in HbA1c and Continuous Glucose Monitoring (CGM) Metrics in Older Adults with Type 2 Diabetes.

Clinical chemistry·2026
Same journal

TSH Pediatric Reference Intervals: Lack of CALIPER Applicability to US-Based Populations.

Clinical chemistry·2026
Same journal

Rapid Detection of Hemoglobinopathy Variants Using One-Step Library Preparation and Nanopore Sequencing.

Clinical chemistry·2026
See all related articles

This study validated a bicarbonate calculation formula using blood gas analysis and two CO2 measurement methods. Results showed high correlation, but identified a positive bias in one method due to organic acids.

Area of Science:

  • Clinical Chemistry
  • Biomedical Analysis
  • Acid-Base Balance

Background:

  • Accurate bicarbonate measurement is crucial for diagnosing acid-base disorders.
  • Existing methods for determining bicarbonate levels can be subject to analytical interferences.
  • The relationship between calculated and measured bicarbonate requires validation across different analytical platforms.

Purpose of the Study:

  • To evaluate the accuracy of a specific formula relating pK' to calculated and measured bicarbonate.
  • To compare bicarbonate values derived from blood gas analysis with those obtained from two different CO2 analyzers (Ektachem 700 and SMA 6/60).
  • To identify potential biases in CO2 measurement methods, particularly the Ektachem 700.

Main Methods:

  • Analysis of 500 blood samples using a Corning 168 blood-gas analyzer for pH and PCO2.

Related Experiment Videos

  • Simultaneous measurement of total carbon dioxide in 500 venous samples using the Ektachem 700 potentiometric analyzer.
  • Determination of total carbon dioxide in 415 arterial and venous samples using the SMA 6/60 colorimetric analyzer.

    • Main Results:

    • High coefficients of determination (r2 = 0.86 and 0.96) were observed between calculated and measured bicarbonate values in both studies.
    • A positive bias was confirmed for the Ektachem method, attributed to interfering organic acids.
    • The SMA 6/60 analysis revealed a significant decrease in pK' for patients with metabolic acidosis.

    Conclusions:

    • The validated formula accurately relates calculated and measured bicarbonate, supporting its use in clinical settings.
    • The Ektachem 700 method exhibits a positive bias, necessitating caution in interpreting results, especially in the presence of organic acids.
    • Metabolic acidosis is associated with a decreased pK', as indicated by SMA 6/60 analysis, highlighting the importance of accurate CO2 measurements.