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Updated: Oct 23, 2026

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
Published on: March 14, 2013
Solution-based indirect affinity selection mass spectrometry--a general tool for high-throughput screening of
Thomas N O'Connell1, Jason Ramsay, Steven F Rieth
1Center of Chemistry Innovation and Excellence and ‡Research Informatics, Pfizer, Inc. , Groton, Connecticut 06340, United States.
Abstract:
We show here that an automated solution-based affinity selection mass spectrometry (ASMS) system can be built exclusively from commercially available parts. The value of this technology lies in the throughput (~1 × 10(5) compounds/day) coupled with a low hit rate. The system, being a binding assay, requires little development time yielding a fast timeline between target availability and hit identification. In addition, the use of exact mass simplifies the hit identification. We demonstrate this system using carbonic anhydrase as the target and a library of 144,000 proprietary compounds.
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