Related Experiment Video
Updated: Apr 26, 2026

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
Published on: June 28, 2024
Synthesis of water-soluble CdSe quantum dots with various fluorescent properties and their application in immunoassay
1Institute of Biochemistry, National Academy of Sciences, P.Sevak str.5/1, Yerevan, 0014, Georgia, vgasparyan@excite.com.
Abstract:
Effects of various factors on synthesis and fluorescent properties of CdSe quantum dots were studied. It was shown that variation of pH, stabilizer and concentration of precursors brings to obtaining of quantum dots with various fluorescent properties. The nanoparticles prepared were conjugated with rabbit antibodies to C-Reactive protein and C-Reactive protein for competitive immunoassay for determination of CRP. It was shown that interaction of these dots as a result of antigen-antibody reaction brings to resonance energy transfer and these changes in fluorescence spectra correlate with concentration of CRP. This approach permits to determine CRP in range between 4-100 ng.

