Related Experiment Video
Updated: Aug 9, 2026
09:41
Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas
Published on: April 1, 2014
A simplified method for purification of annexin V from human placenta
G G Poghosyan1, V Z Melkonyan, M V Mikaelyan
1Institute of Biochemistry, NAS of Armenia, Yerevan, Republic of Armenia.
Preparative Biochemistry & Biotechnology
|August 15, 2003
Abstract:
A simplified procedure for purification of annexin V from human placenta was developed. At first, the protein was separated from other proteins in membrane bound form in the presence of Ca2+, then was extracted with EDTA and purified by affinity chromatography on PAAG-immobilized phosphatidylserine. The purified protein gave a single band with a molecular weight of 35,000 in SDS-PAGE.
