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Serum paraoxonase 1 activity and oxidative stress in pediatric patients with pulmonary tuberculosis
Emel Torun1, Ahmet Hakan Gedik, Erkan Cakir
1Department of Pediatrics, Bezmialem Vakif University, Istanbul, Turkey.
Insights
Children with pulmonary tuberculosis exhibit elevated oxidative stress, indicated by higher total oxidant status (TOS) and oxidative stress index (OSI), and lower paraoxonase 1 (PON1) levels compared to healthy children.
Area of Science:
- Biochemistry
- Pediatrics
- Infectious Diseases
Background:
- Oxidative stress plays a role in various diseases.
- Paraoxonase 1 (PON1) is an enzyme with antioxidant properties.
- Pulmonary tuberculosis (TB) is a significant global health concern in children.
Purpose of the Study:
- To compare oxidative stress markers and PON1 levels in children with pulmonary TB versus healthy controls.
- To investigate the relationship between demographic factors and oxidative stress in pediatric TB patients.
Main Methods:
- Forty children with pulmonary TB and 40 healthy controls were recruited.
- Serum total antioxidant status (TAS), total oxidant status (TOS), and PON1 levels were measured.
- Oxidative stress index (OSI) was calculated.
Main Results:
- Pediatric TB patients showed significantly lower TAS and PON1 levels compared to controls.
- TOS and OSI levels were significantly higher in children with pulmonary TB.
- Lower PON1 levels correlated with TAS and OSI, but not anthropometric parameters.
Conclusions:
- Children with pulmonary TB experience increased oxidative stress.
- Reduced PON1 levels may be associated with heightened oxidative stress in pediatric TB.
Objectives:
The aim of this study was to determine the oxidative stress and paraoxonase 1 (PON1) levels in children with pulmonary tuberculosis (TB) compared to healthy controls, and to examine the association of demographical with oxidative stress.
Subjects And Methods:
Forty children diagnosed with pulmonary TB and 40 age- and gender-matched healthy controls were enrolled in the study. Serum total antioxidant status (TAS), total oxidant status (TOS) and PON1 levels were measured. The oxidative stress index (OSI) was calculated to indicate the degree of oxidative stress.
Results:
The TAS levels were lower (1.73 ± 0.5 vs. 2.54 ± 1.2 μmol Trolox Eq/l) while TOS levels were significantly higher (26.9 ± 14.4 vs. 13.4 ± 7.7 μmol H2O2 Eq/l) in the TB group than in the controls (p < 0.001). The OSI was significantly higher in the TB group than in the controls (21.2 ± 5.1 vs. 6.5 ± 4.9 units, p = 0.006). Serum PON1 levels were significantly lower in the TB group than in the controls (14.2 ± 13.2 vs. 28.4 ± 17.3 U/l, p < 0.001). The lower PON1 levels correlated with TAS and OSI levels but not with anthropometric parameters (r = 0.264, p = 0.018 and r = -0.255, p = 0.023, respectively).
Conclusion:
The TOS and OSI levels were higher and the TAS and PON1 levels were lower in pediatric patients with pulmonary TB when compared to healthy controls. This indicates greater oxidative stress in the patients.
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