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ECD of tyrosine phosphorylation in a triple quadrupole mass spectrometer with a radio-frequency-free
Valery G Voinov1, Samuel E Bennett, Joseph S Beckman
1Department of Chemistry, Oregon State University, Corvallis, OR, 97333, USA, valery.voinov@orst.edu.
Abstract:
A radio frequency-free electromagnetostatic (EMS) cell devised for electron-capture dissociation (ECD) of ions has been retrofitted into the collision-induced dissociation (CID) section of a triple quadrupole mass spectrometer to enable recording of ECD product-ion mass spectra and simultaneous recording of ECD-CID product-ion mass spectra. This modified instrument can be used to produce easily interpretable ECD and ECD-CID product-ion mass spectra of tyrosine-phosphorylated peptides that cover over 50% of their respective amino-acid sequences and readily identify their respective sites of phosphorylation. ECD fragmentation of doubly protonated, tyrosine-phosphorylated peptides, which was difficult to observe with FT-ICR instruments, occurs efficiently in the EMS cell.
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