Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Methods of Medium Optimization01:28

Methods of Medium Optimization

69
Optimizing growth media enhances microbial proliferation and maximizes product yield. Statistical experimental design methodologies provide structured and reproducible approaches, offering progressively higher levels of robustness and efficiency.The One-Factor-at-a-Time (OFAT) MethodThe One-Factor-at-a-Time (OFAT) method involves adjusting a single variable while keeping all others constant. However, it cannot detect interactions between variables, often leading to suboptimal outcomes when...
69
Mechanistic Models: Compartment Models in Algorithms for Numerical Problem Solving01:29

Mechanistic Models: Compartment Models in Algorithms for Numerical Problem Solving

438
Mechanistic models play a crucial role in algorithms for numerical problem-solving, particularly in nonlinear mixed effects modeling (NMEM). These models aim to minimize specific objective functions by evaluating various parameter estimates, leading to the development of systematic algorithms. In some cases, linearization techniques approximate the model using linear equations.
In individual population analyses, different algorithms are employed, such as Cauchy's method, which uses a...
438
One-Compartment Open Model: Wagner-Nelson and Loo Riegelman Method for ka Estimation01:24

One-Compartment Open Model: Wagner-Nelson and Loo Riegelman Method for ka Estimation

1.5K
This lesson introduces two critical methods in pharmacokinetics, the Wagner-Nelson and Loo-Riegelman methods, used for estimating the absorption rate constant (ka) for drugs administered via non-intravenous routes. The Wagner-Nelson method relates ka to the plasma concentration derived from the slope of a semilog percent unabsorbed time plot. However, it is limited to drugs with one-compartment kinetics and can be impacted by factors like gastrointestinal motility or enzymatic degradation.
On...
1.5K
Titrimetric Methods: Types and Commonly Used Strategies01:08

Titrimetric Methods: Types and Commonly Used Strategies

2.2K
In chemistry, titrimetric methods are broadly classified into three types: volumetric, gravimetric, and coulometric. Volumetric titrations involve measuring the volume of a titrant of known concentration that is required to react completely with an analyte. In gravimetric titrations, the standard solution reacts with the analyte to form an insoluble precipitate, which is filtered, dried, and weighed. In coulometric titrations, current is applied to an electrochemical reaction until the reaction...
2.2K
A Single-Component System01:24

A Single-Component System

68
In the field of chemistry, the terms "component" and "phase" hold significant importance. A component refers to a chemically distinct substance in a system that has specific properties. It is chemically homogeneous, meaning it has the same properties throughout. For example, in a mixture of salt and water, both salt and water are considered separate components because they have different chemical properties.On the other hand, a phase is a form of matter that has a consistent chemical...
68
Model-Independent Approaches for Pharmacokinetic Data: Noncompartmental Analysis00:59

Model-Independent Approaches for Pharmacokinetic Data: Noncompartmental Analysis

422
Noncompartmental analyses offer an alternative method for describing drug pharmacokinetics without relying on a specific compartmental model. In this approach, the drug's pharmacokinetics are assumed to be linear, with the terminal phase log-linear. This assumption allows for simplified analysis and interpretation of the drug's behavior in the body.
One important characteristic of noncompartmental analyses is that drug exposure increases proportionally with increasing doses. This...
422

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparing the Degradation Pathways of Hydrochlorothiazide and Sulfamethoxazole Using Ozone, Anodic Oxidation, and Electro-Fenton Processes.

ACS ES&T water·2026
Same author

Hydrogen supplementation enhances microbial removal of selected organic micropollutants, reduces associated ecotoxicity, and improves nutrient removal in domestic wastewater effluent.

Water research·2026
Same author

Uncertainty-driven model-based search methods for method development in liquid chromatography.

Journal of chromatography. A·2026
Same author

A Protocol for Characterizing Comprehensive Two-Dimensional Liquid Chromatography Systems.

Journal of separation science·2026
Same author

Elucidating the underlying mechanism of the total breakthrough phenomenon in reversed-phase liquid chromatography.

Journal of chromatography. A·2026
Same author

A hydrophilic interaction liquid chromatography-tandem mass spectrometry method for the quantification of intracellular cystine and its application in cystinosis research.

Analytical and bioanalytical chemistry·2026
Same journal

Impurity profiling of lipid-conjugated oligonucleotides using reversed-phase with and without ion-pair reagents and hydrophilic interaction liquid chromatography.

Journal of chromatography. A·2026
Same journal

Preparation of magnetic zwitterionic covalent organic frameworks for rapid simultaneous extraction of hydrophilic and hydrophobic organophosphates from environmental waters coupled with UHPLC-MS/MS determination.

Journal of chromatography. A·2026
Same journal

Analysis of organic and inorganic acids in biomass pyrolysis process samples by ion chromatography-mass spectrometry.

Journal of chromatography. A·2026
Same journal

Separation and enrichment of phages at the interface between two phases in a green solvent-based sugaring-out extraction system.

Journal of chromatography. A·2026
Same journal

Advances and perspectives in Oligo(dT) Affinity chromatography for mRNA capture: Resins, ligands and process intensification.

Journal of chromatography. A·2026
Same journal

Ion chromatography: Current strengths, key limitations, and future trends.

Journal of chromatography. A·2026
See all related articles

Related Experiment Video

Updated: Apr 26, 2026

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
06:24

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Published on: December 15, 2017

13.5K

Enhanced selectivity and search speed for method development using one-segment-per-component optimization strategies.

Eva Tyteca1, Kim Vanderlinden1, Maxime Favier2

  • 1Department of Chemical Engineering (CHIS-IR), Vrije Universiteit Brussel, Belgium.

Journal of Chromatography. A
|July 21, 2014
PubMed
Summary
This summary is machine-generated.

A new gradient optimization algorithm for liquid chromatography significantly reduces separation time by adjusting the gradient slope after each component elutes. This "one-segment-per-group-of-components" strategy offers faster, more efficient separations compared to traditional methods.

Keywords:
Gradient optimizationLSS modelMethod developmentMulti-segment gradientsNon-linear retention modelRetention modeling

More Related Videos

A Workflow for Lipid Nanoparticle LNP Formulation Optimization using Designed Mixture-Process Experiments and Self-Validated Ensemble Models SVEM
13:54

A Workflow for Lipid Nanoparticle LNP Formulation Optimization using Designed Mixture-Process Experiments and Self-Validated Ensemble Models SVEM

Published on: August 18, 2023

6.4K
Author Spotlight: Accelerating Discovery in Microporous Material Chemistry
07:20

Author Spotlight: Accelerating Discovery in Microporous Material Chemistry

Published on: October 6, 2023

4.1K

Related Experiment Videos

Last Updated: Apr 26, 2026

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
06:24

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Published on: December 15, 2017

13.5K
A Workflow for Lipid Nanoparticle LNP Formulation Optimization using Designed Mixture-Process Experiments and Self-Validated Ensemble Models SVEM
13:54

A Workflow for Lipid Nanoparticle LNP Formulation Optimization using Designed Mixture-Process Experiments and Self-Validated Ensemble Models SVEM

Published on: August 18, 2023

6.4K
Author Spotlight: Accelerating Discovery in Microporous Material Chemistry
07:20

Author Spotlight: Accelerating Discovery in Microporous Material Chemistry

Published on: October 6, 2023

4.1K

Area of Science:

  • Analytical Chemistry
  • Chromatography

Background:

  • Linear gradient programs are common in reversed-phase liquid chromatography for enhanced selectivity.
  • Multi-linear gradient programs offer greater separation power but are underutilized.
  • Advancements in instrument gradient-conformity necessitate improved gradient design algorithms.

Purpose of the Study:

  • To explore a novel multi-segment gradient optimization algorithm.
  • To introduce the "one-segment-per-group-of-components" strategy for gradient design.
  • To evaluate the efficiency and performance of this new approach.

Main Methods:

  • Developed and experimentally applied a "one-segment-per-group-of-components" gradient optimization strategy.
  • Adjusted gradient slope after the elution of each sample component.
  • Validated findings through in silico studies on various sample compositions.

Main Results:

  • Achieved an average reduction in separation time of approximately 40% compared to single linear gradients.
  • The new method performed comparably to or better than commercial multi-segment optimization software.
  • In silico studies confirmed the advantages across diverse sample sets (10 and 20 components).

Conclusions:

  • The "one-segment-per-group-of-components" strategy offers a significant improvement in liquid chromatography separation efficiency.
  • This auto-guided gradient approach reduces analysis time and enhances performance.
  • The method provides a faster and more effective alternative to traditional gradient design techniques.