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Restriction map of mycobacteriophage D29 and its deletion mutant F5
R Lazraq1, J Moniz-Pereira, S Clavel-Sérès
1Unité de Tuberculose et Mycobactéries, Paris, France.
Acta Leprologica
|January 1, 1989
Abstract:
A physical map of mycobacteriophage D29 was constructed, including positions for 25 restriction sites for 9 endunocleasic enzymes. D29 DNA contains about 48 150 bp. Analysis of a deletion mutant (F5) has allowed to determine the location of two non essential regions in the genome, allowing further insertion of foreign genes and construction of cosmids.