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A fluorescent nucleic acid nanodevice quantitatively images elevated cyclic adenosine monophosphate in membrane-bound
Suruchi Sharma1, Anisha Zaveri, Sandhya S Visweswariah
1Department Biophysics and Biochemistry, National Centre for Biological Sciences, UAS-GKVK, Bellary Road, Bangalore-, 560065, India.
Small (Weinheim an Der Bergstrasse, Germany)
|July 22, 2014
Abstract:
cAMPhor: In the presence of cAMP, cAMPhor folds into a structure that binds DFHBI (green), increasing its fluorescence, while Alexa 647 (red) functions as a normalizing dye. It can thus be used to spatially image cAMP quantitatively in membrane-bound compartments.
