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Updated: Apr 26, 2026

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Biotechnological approach towards a highly efficient production of natural prostaglandins
J C Guder1, M Buchhaupt, I Huth
1Biochemical Engineering, DECHEMA Research Institute, Theodor-Heuss-Allee 25, 60486, Frankfurt/Main, Germany.
Abstract:
Prostaglandins (PGs) act as potent local hormones in nearly all tissues of the human body and are used for various medical applications. Heterologous expression of PG endoperoxide H-synthase from the alga, Gracilaria vermiculophylla, into E. coli and the application of this strain in biotransformation experiments resulted in a highly efficient conversion of arachidonic acid (ARA) yielding up to 130 mg natural PGs l(-1) in a laboratory scale approach. Detailed analyses of the products and production kinetics were performed, confirming a rapid conversion of ARA to PGs.
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