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Updated: Apr 26, 2026

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
Published on: December 16, 2013
Copper(II) complexes with 4-hydroxyacetophenone-derived acylhydrazones: synthesis, characterization, DNA binding and
Ramazan Gup1, Cansu Gökçe1, Selçuk Aktürk2
1Department of Chemistry, Mugla Sıtkı Koçman University, 48100 Mugla, Turkey.
Abstract:
Two new Cu(II) complexes of Schiff base-hydrazone ligands, hydroxy-N'-[(1Z)-1-(4-hydroxyphenyl)ethylidene]benzohydrazide [H₃L(1)] and ethyl 2-(4-(1-(2-(4-(2-ethoxy-2-oxoethoxy)benzoyl)hydrazono)ethyl)phenoxy)acetate (HL(2)) have been synthesized and then characterized by microcopy and spectral studies. X-ray powder diffraction illustrates that [Cu(L(2))₂] complex is crystalline in nature whereas [Cu(H₂L(1))₂]·2H₂O has an amorphous structure. Binding of the copper complexes with Calf thymus DNA (CT-DNA) has been investigated by UV-visible spectra, exhibiting non-covalent binding to CT-DNA. DNA cleavage experiments have been also investigated by agarose gel electrophoresis in the presence and absence of an oxidative agent (H₂O₂). The effect of complex concentration on the DNA cleavage reaction has been also studied. Both copper complexes show nuclease activity, which significantly depends on concentrations of the complexes, in the presence of H₂O₂ through oxidative mechanism whereas they slightly cleavage DNA in the absence an oxidative agent.
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