Electrochemical enzymatic biosensor with long-term stability using hybrid mesoporous membrane

Area of Science:

• Biochemical sensors
• Nanomaterials science
• Analytical chemistry

Background:

• Acetylcholinesterase (AChE) is crucial for neurotransmission and is a target for pesticide inhibition.
• Developing stable and sensitive biosensors for pesticide detection remains a significant challenge.
• Mesoporous silica materials offer unique properties for enzyme immobilization.

Purpose of the Study:

• To develop a novel acetylcholinesterase-immobilized sensor unit using hybrid mesoporous silica membranes (F127-MST).
• To evaluate the sensor's performance in detecting acetylcholine and organophosphorus pesticides.
• To create a working prototype enzyme sensor for practical pesticide analysis.

Main Methods:

• Enzyme immobilization of acetylcholinesterase within F127-MST hybrid mesoporous silica membranes.
• Integration with tetracyanoquinodimethane for enhanced detection capabilities.
• Fabrication and testing of a working prototype enzyme sensor.

Main Results:

• The F127-MST encapsulated AChE sensor demonstrated high sensitivity for detecting acetylcholine and organophosphorus pesticides (dichlorvos, aldicarb, parathion).
• The working prototype achieved detection limits comparable to or better than existing methods.
• Enzyme encapsulation within the silica nanospace significantly improved enzyme stability.

Conclusions:

• Acetylcholinesterase immobilized in F127-MST represents a practical sensor with high sensitivity, reusability, and storage stability.
• The developed sensor offers a promising platform for sensitive and reliable pesticide monitoring.
• This approach enhances enzyme stability and sensor performance for environmental and agricultural applications.