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Related Experiment Videos

Calcium buffer injections block fucoid egg development by facilitating calcium diffusion.

J E Speksnijder1, A L Miller, M H Weisenseel

  • 1Marine Biological Laboratory, Woods Hole, MA 02543.

Proceedings of the National Academy of Sciences of the United States of America
|September 1, 1989
PubMed
Summary
This summary is machine-generated.

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Calcium ion influx is crucial for fucoid egg polarity and tip growth. Injecting specific calcium buffers disrupted this process, indicating a high calcium concentration is essential at the incipient tip.

Area of Science:

  • * Cell biology
  • * Developmental biology
  • * Biophysics

Background:

  • * Polarity establishment in fucoid eggs is critical for development.
  • * A localized high calcium zone is hypothesized to be essential for tip growth initiation.
  • * The precise calcium concentration required at the incipient tip remains unquantified.

Purpose of the Study:

  • * To test the hypothesis that a steady influx of calcium ions establishes a high calcium zone necessary for fucoid egg polarity and tip growth.
  • * To determine the optimal calcium concentration range for incipient tip formation.
  • * To investigate the role of calcium gradients in fucoid egg development.

Main Methods:

  • * Injection of seven different 1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA)-type calcium buffers into Pelvetia eggs.

Related Experiment Videos

  • * Measurement of critical inhibitory concentrations of BAPTA buffers required to block outgrowth and cell division.
  • * Derivation and application of a facilitated diffusion model to analyze calcium gradients.

    • Main Results:

    • * BAPTA buffer injections blocked outgrowth and cell division for up to two weeks.
    • * The critical inhibitory concentration of BAPTA buffers was lowest for dissociation constants (Kd) of 4-5 x 10(-6) M.
    • * Data fit a facilitated diffusion model assuming a normal cytosolic free calcium level of approximately 7 microM at the incipient tip.

    Conclusions:

    • * A high calcium concentration (around 7 microM) is essential for initiating tip growth and polarity in fucoid eggs.
    • * The precise buffering capacity of calcium buffers is critical for their inhibitory effects.
    • * The study provides quantitative evidence supporting the role of calcium gradients in fucoid egg development.