Microarray immobilization of biomolecules using a fast trans-cyclooctene (TCO)-tetrazine reaction
Ping Wang1, Zhenkun Na, Jiaqi Fu
1Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institute of City University of Hong Kong, Shenzhen, 518057, P. R. China. hongysun@cityu.edu.hk.
Chemical Communications (Cambridge, England)
|July 24, 2014
Abstract:
By anchoring 1,2,4,5-tetrazine-containing biomolecules onto trans-cyclooctene (TCO)-functionalized slides, a site-specific microarray immobilization approach is described in this study. Compared with existing immobilization methods, our approach offers several distinctive features, including fast kinetics and high chemoselectivity.
