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Related Experiment Videos

Human-parathormone assay for use in dogs: validation, sample handling studies, and parathyroid function testing.

A G Torrance1, R Nachreiner

  • 1Department of Small Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing 48824.

American Journal of Veterinary Research
|July 1, 1989
PubMed
Summary

A validated immunoradiometric assay accurately measures canine parathormone (PTH). Proper sample handling, including refrigeration and freezing, is crucial for preserving PTH stability, while infusions of EDTA and calcium effectively test parathyroid function in dogs.

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Area of Science:

  • Veterinary Endocrinology
  • Clinical Pathology
  • Assay Validation

Background:

  • Accurate measurement of parathormone (PTH) is essential for diagnosing and managing endocrine disorders in dogs.
  • Existing commercial PTH assays may not be suitable for canine samples, necessitating validation and optimization.
  • Understanding sample handling and storage conditions is critical for reliable PTH testing.

Purpose of the Study:

  • To competitively validate ten commercially available parathormone (PTH) assays for canine use.
  • To identify the most suitable assay for accurate canine PTH measurement.
  • To evaluate sample handling and storage effects on PTH stability and to assess parathyroid function testing methods in dogs.

Main Methods:

  • Validation of ten PTH assays using dilutional parallelism, coefficient of variation, and sensitivity analyses.

Related Experiment Videos

  • Testing assay performance with canine samples and responses to calcium and EDTA infusions.
  • Investigating sample stability under various storage conditions (temperature, time) and freeze-thaw cycles.
  • Conducting parathyroid function tests in dogs using infusions of disodium EDTA and calcium gluconate, with renal function monitoring.

    • Main Results:

    • A 2-site immunoradiometric assay for intact human-PTH demonstrated superiority for estimating canine-PTH and met validity criteria.
    • Serum and plasma samples showed significant PTH loss at 24°C within 2-24 hours, but not at 6°C.
    • Serum samples stored at 6°C and 37°C deteriorated by 35% and 100% within a week, respectively, while frozen samples (-20°C and -70°C) remained stable for 4 weeks.
    • EDTA infusion significantly increased PTH levels (P < 0.001), and calcium gluconate infusion significantly decreased PTH levels (P < 0.005) in dogs, with no adverse effects or changes in renal function.

    Conclusions:

    • A specific 2-site immunoradiometric assay is validated as a reliable method for intact canine parathormone (PTH) measurement.
    • Refrigeration (6°C) or freezing (-20°C or -70°C) is recommended for preserving canine serum/plasma PTH stability.
    • Infusion protocols using EDTA and calcium are effective and safe for assessing canine parathyroid gland function.