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Published on: July 2, 2013
Lytic effect of Pseudomonas aeruginosa elastase on gram-positive and -negative bacteria
M Kaur1, M Gupta, K K Tripathi
1Department of Microbiology, Panjab University, India.
Abstract:
Elastase of Pseudomonas aeruginosa P3 caused lysis of freshly grown cells of Gram-positive (Staphylococcus aureus and S. epidermidis) and Gram-negative (Proteus vulgaris and Salmonella typhi) bacteria. Heat killed and lyophilized Gram-negative bacteria showed higher sensitivity to elastase. Both Gram-negative and Gram-positive bacteria were lysed maximally by elastase at pH 8.0. At this pH, the activity of elastase was maximal in Tris-HCl and glycine-NaOH buffers followed by Tris-maleate and Cacodylate buffers.
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