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Volumetric absorptive microsampling: a dried sample collection technique for quantitative bioanalysis.

Philip Denniff1, Neil Spooner

  • 1Bioanalytical Science and Toxicokinetics, Drug Metabolism and Pharmacokinetics, GlaxoSmithKline Research and Development , Ware, Hertfordshire SG12 0DP, United Kingdom.

Analytical Chemistry
|July 25, 2014
PubMed
Summary
This summary is machine-generated.

Volumetric absorptive microsampling (VAMS) offers a novel method for dried blood samples in bioanalysis. This technique overcomes limitations of traditional dried blood spots (DBS), ensuring consistent blood volume collection.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Pharmacokinetics

Background:

  • Dried blood spot (DBS) sampling is widely used but faces challenges with area bias and homogeneity.
  • Conventional DBS methods can lead to inaccurate quantitative bioanalysis due to sampling inconsistencies.
  • Novel microsampling techniques are needed to improve the reliability of dried blood sample collection.

Purpose of the Study:

  • To evaluate the performance of Volumetric Absorptive Microsampling (VAMS) for quantitative bioanalysis.
  • To assess VAMS's ability to overcome the limitations of conventional DBS.
  • To establish best practices for VAMS sample collection, considering potential abuse scenarios.

Main Methods:

  • VAMS devices were tested for blood absorption volume and variability across a wide hematocrit range (20-70%).
  • Tip-to-tip variability and plasma component absorption were analyzed.
  • Simulated abuse scenarios were used to develop collection recommendations.

Main Results:

  • VAMS consistently absorbs a fixed blood volume (approximately 10 μL) within 2-4 seconds.
  • Volume variation was less than 5% across the tested hematocrit range, with low tip-to-tip variability.
  • No evidence of selective plasma component absorption was observed.

Conclusions:

  • VAMS provides a reliable and reproducible method for obtaining dried blood samples for quantitative bioanalysis.
  • The VAMS technique effectively addresses the area bias and homogeneity issues inherent in conventional DBS.
  • Developed best practices enhance the integrity and usability of VAMS samples in various research and clinical settings.